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> Trypsin edta 0.25% solution - 500ML
Trypsin edta 0.25% solution - 500ML
Trypsin may be used to remove adherent cells from a culture surface. Cells are most commonly removed from the culture substrate by treatment with trypsin, or trypsin EDTA. Incubating cells with too high of a trypsin concentration for too long a time will damage cell membranes and kill the cells. If unsure about the concentration of trypsin to use, use a lower concentration. There may be lot to lot variation in dissociation times which is to be expected since the enzymatic activity of each lot will differ..
* 2.5 g/L irradiated porcine trypsin
* 0.2 g/L EDTA 4Na in Hank s Balanced Salt Solution
* Without calcium and magnesium
