Chicago Industrial Reuse and Parts Blog-Newsgroup > Cook County
> New
> Agricultural
> Engines
> New haematoxylin 50 ml -
New haematoxylin 50 ml -
Haematoxylin, hematoxylin, Natural Black 1, or C.I. 75290 is extracted from the wood of the logwood tree. When oxidized it forms haematein, a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminium-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.
Its CAS number is [517-28-2] and its SMILES structure is OC(C(O)=C4)=C C1=C4CC3(O) C1C2=CC=C(O) C(O)=C2OC3.
Haematoxylin and eosin stain is one of the most commonly used stains in histology. It is a permanent stain as opposed to temporary stains (e.g. iodine solution in KI).
Other common stain is phosphotungstic acid haematoxylin, a mix of haematoxylin with phosphotungstic acid.
In 1970s, due to clear felling of forests in Brazil and Central America, there was a shortage of logwood and therefore of haematoxylin. Its price went to record heights, which affected the cost of diagnostic histopathology, and prompted a search for alternative nuclear stains. Before the use of any alternatives became firmly established, haematoxylin returned to the market, though at a higher price, and resumed its place in histopathology. There were several dyes recommended as replacements: Celestine blue B (CI 51050), Gallocyanin (CI 51030), Gallein (CI 45445) and Solochrome cyanin (CI 43820). All four used Fe(III) as the mordant. Another alternative is the red dye brazilin, which differs from haematoxylin by only one hydroxyl group.
Ready made and ready for immediate use. Great price for this prepared reagent.
